steve b replied: "They will get the correct medication and dose in due course, which is why, in the early stages of diagnosis, they ask you back regularly for check-ups."
hi there ? i have questions? Currently Ezetimibe with attorvastatin is used to control LDL in particular and l Currently Ezetimibe with attorvastatin is used to control LDL in particular and lipids in general. In my case, My Lp(a)is 55 while lipid range is normal. Is it necessary to add Ezetimibe to statin for Lp(a) in the drug regimen ? 2. Diruitic like Indapamide 1.5 SR or HCTZ 12.5 is contraindicated in person having high uric acid levels without gouty manifestations and on allopurinol 300 ? 4. What are the pssible options for me who is on amlodipine 10 mg Losartan 100 and Atenolol-100. Doctor wants me to delete Amlodipine because of pedal edema- Mild incompetent sapheno femoral junction. If Amlodipine is deleted, what are other options ? Does combinations of Ramipril 10 + Losartan 100 + Atenolol 100 take care of amlodipine ? I am male 50 hypertensive with obesity 105 kg. Non diabetic non smoker non alcoholic stricy vegitarian. Please guide. 5. Is Nicotinic Acid or Fenofibrate helpful in increasing HDL.? I am a brisk walker but unable to increase my HDL. I shall be obliged to receive your gu
How can i make the peak for amlodipine appear using HPLC? How can i make the peak for amlodipine appear using HPLC?
i'm still new in HPLC. I'm trying to analyze amlodipine at the concentrations of 100 ng/ml to 1000 ng/ml (100 ng/ml, 250, 500 and 1000).
here are some additional information bout the HPLC that i'm using:
column: 46-150 mm
wavelength is set at 240 - 362
running time: 1 hour
mobile phase: ACN + KH2PO4 (37:43 V/V)
i've tried using 10 mg/ml and the peak appeared at 6.34 mins but i can't get it to appear at other concentrations that i've mentioned above. Can somebody help me pls?
here's some more details that i've forgotten to put.
injection volume is 100 µL
column is C18
i've tried diluting the Amlodipine from the stock solution (1mg/ml) using mobile phase and the peak does appear.
But when i tried using MeOH...there was no peak except for the solvent peak.
the pH of the buffer is 3.5
apoorapothecary replied: "Okay - there's some key details missing in your question. You look to have a buffer set up - what pH are you at?
The column - is it a C18? Column temperature?
What instrument are you using?
All the details are pretty important if you want to improve the chromatography.
If you're getting a consistent peak for 10mg/ml, here's what I think you might do to possibly improve your results.
Wavelength - you have a range listed - are you capturing with diode array? If its a single-wavelength, you should likely have the wavelength setting at 360 - 366, roughly where the max is. If its a Agilent/DAD system, run a minimum of a 8nm bandwidth, turn off the reference bandwidth, and have the slit set to at least 4. Keep the defaults for most of the rest.
Injection volume - you should be injecting at least 10µL - you can probably go up to 50µL without a peak distortion problem, if your diluent is close to the mobile strength.
Most importantly - CHANGE ONE THING AT A TIME! Don't make the mistake of rushing things through, particularly as a newb to HPLC. You want to learn the nuances of chromatography, and what effect changes to the system have on performance. Make those changes in bulk, and you'll never know for sure what you did to develop your chromatography. I know of people who have been in the industry for years who still "shotgun develop" the method, and they're always in the dark as to the best ways to efficiently develop a high-quality method.
Good luck!
Okay, given your additional information, 100µl injection using methanol as a diluent may be adversely affecting your separation. With a 4.6 column, you shouldn't be injecting more than 50µl (give or take given analyte mass), and even there, you should be approximating the initial mobile phase conditions (you can omit the buffer - that's okay). Compare your void volume peaks between the one injected in mobile phase and the one in methanol, and one of a methanol blank alone for evidence of the peak washing out in your void.
Did you check to see if your sample can dissolve in an acetonitrile:water mix approximating your initial conditions?
Also, I forgot - when I'm running above 350nm, I make sure both UV and vis lights are on on my detector, just to be sure, since visible starts around 380, it couldn't hurt!"
Merlin's Feline replied: "Based on the data I could find...most HPLC analyses of amlodipine use dynamic ranges of 1 to 90 micrograms /mL and a wavelength of 254 nm. I suspect that the molar absorptivity is around 20,000 M^-1cm^-1 which means that an absorbance of 0.05 would be equivalent to about
2.5 X 10^-6 M = 2.5 micromolar = ~ 1.02 ug/mL Try usng the above dynamic range for your standards and adjusting the sensitivity of your UV detector to 0.1 AUFS ad maybe even 0.05 AUFS if the baseline is fairly flat and you will have a better S/N ratio"
medicine question ? I am 38 years old , healthy guy i only have high blood pressure i am taking two medicine lisinopril 10 mg and amlodipine 5 mg and my blood pressure before medicine was 175/95 and now in doctor office 110/62 .
all my blood tests normal
my question is does amlodipine lower cholestrol level ,uric acid that coz gout and glouces or its increase ? is the both medicine safe? doctor said lisiopirl isvery good medicine and its protect ur kidney and
hope to find a good answer from educated people
10 points for best answer
TONY C replied: "You're in good shape..Stay on them.
Neither lowers cholesterol or uric acid and both are quite safe."

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